Protein Characterization

ABT also has expertise in protein characterization and assay development, both to monitor the recovery, purity, and properties of protein products and for downstream quality control and analysis.

Characterization assays include:

• Biomolecular Interactions (Kd, rate constants)
  Immobilized macromolecule or ligand (Biacore)
  Solution dissociation constants (antibodies)
• Gel Electrophoresis
  SDS-PAGE
  Isoelectric focusing
  Western Blots
• ELISA Assay Development
• Enzymatic Assay Development
• Chromatographic Assays
  Size Exclusion
  Reverse Phase
  Ion Exchange
• Mass Spectrometry Analysis, Mapping, and Sequencing
  ABT will prepare the samples, arrange for a contractor to do the mass spectrometry analysis, and assist in the interpretation of the data.