ABT has considerable experience at gene expression in bacterial systems, including the modification of synthetic genes for improved expression and solubility in bacteria. Our GEOS approach can ensure that more genes express well and produce soluble protein products.
Components of recombinant expression systems can be addressed to improve expression of 'difficult' heterologous genes. ABT synthetic genes are designed from the start not only to enhance the expression of multiple recombinant proteins in bacterial systems, but also to ease the manipulation and reduce the cost of modification of these genes as they advance through the development process.
GEOS incorporates novel approaches to enhance the translation efficiency of the heterologous genes of interest. mRNA sequences vary between species and these variations can have significant effects on the recombinant proteins produced by standard expression systems. ABT synthetic biology approach achieves substantial improvements in expression levels through subtle mRNA modification.
Taming the Wild Type Gene
Many potential approaches to improving gene expression and solubility require the construction of multiple gene variants, or expression construct variants. The sequences of native genes themselves can often complicate rapid gene manipulation. ABT can modify such genes upfront, without changing the encoded proteins amino acid sequences, to facilitate their downstream manipulation.
Benefits
The use of such 'domesticated' wild type genes with synthetic biology approaches can significantly reduce the cost of downstream manipulations.
ABT's approach can improve the efficiency of moving interesting genes into plants, or performing protein engineering & mutagenesis to increase protein bioactivity.
GEOS addresses expression problems using secretion systems and several alternative secretion approaches.
